军曹鱼的分子遗传特性研究   总被引：8，自引：1，他引：8  

刘楚吾  刘丽  王中铎  黎雨轩 《热带海洋学报》2005,24(1):77-85

采用随机扩增多态性DNA(RAPD)和线粒体DNA(mtDNA)的限制性片段长度多态性(RFLP)分析方法对广东湛江近海军曹鱼Rachycentron canadum的分子遗传学特征进行了研究。RAPD研究结果显示17个引物共检测到119个位点，其中多态位点比例P为80．85％，遗传相似系数S为0．7400，遗传距离D为0．2600，Nei基因多样性指数H为0．3009，Shannon信息指数I为0．4498。结果表明，军曹鱼的遗传多样性比较丰富。用19种识别5、6碱基的限制性内切酶对军曹鱼的mtDNA RFLP进行了分析研究，构建其mtDNA物理图谱；用引物5’-GTG ATC TGA AAA ACC ACC GTT G-3’和5’-AAT AGG AAG TAT CAT TGC GGT TTG ATG-3’扩增线粒体细胞色素b区段，得到稳定的850bp大小的特异性条带，用18种识别4-6碱基的限制性内切酶对扩增片段的限制性长度多态性进行分析，并测定其序列。  相似文献   

绒螯蟹种质资源研究进展   总被引：1，自引：1，他引：1  

张秀梅  柳广东  高天翔 《中国海洋大学学报(自然科学版)》2002,32(4):533-542

本文综述了近年来有关绒螯蟹属在形态学、遗传学和育种学方面的研究进展 ,着重介绍了同工酶、RAPD和 mt DNA技术在绒螯蟹群体遗传学中的应用 ,提出遗传学分类标准的建立是绒螯蟹属分子分类的关键所在。  相似文献   

Microbial Diversity in Nankai Trough Sediments at a Depth of 3,843 m   总被引：6，自引：0，他引：6  

Lina Li  Jean Guenzennec  Peter Nichols  Pierre Henry  Miki Yanagibayashi  Chiaki Kato 《Journal of Oceanography》1999,55(5):635-642

Dense populations of bivalves, primarily Calyptogena sp., were observed at cold seeps of the Nankai Trough. Bacterial input to the sediment was estimated through determination of phospholipid ester-linked fatty acid (PLFA) and DNA profiles. Results indicated a bacterial biomass of 10⁹ cells (g dry wt)^-1 while individual fatty acid profiles revealed a predominance of monounsaturated fatty acids, mainly 18:1 isomers. The presence of these fatty acids can be interpreted to reflect a response to low temperature and a predominance of psychrophilic bacteria. DNA fragments encoding bacterial ribosomal RNA small-subunit sequences (16S rDNA) were amplified by the polymerase chain reaction method using DNA extracted directly from the sediment samples. From the sequencing results, at least 19 kinds of bacterial 16S rDNAs related to mostly the Proteobacteria and a few gram-positive bacteria were identified. These results suggest that the bacterial community in the Nankai Trough sediments consists of mainly bacteria belonging to the Proteobacteria , , and subdivisions. Bacteria belonging to the and subdivisions, which are known to include epibiont and sulfate reducing bacteria, respectively, were mostly detected in the sediment obtained from inside the area of the Calyptogena community, and the -Proteobacteria may function to supply reduced sulfur to bacterial endosymbionts of Calyptogena.  相似文献   

Trends in biomass, density and diversity of North Sea macrofauna   总被引：6，自引：2，他引：6  

Heip  C.; Basford  D.; Craeymeersch  J. A.; Dewarumez  J. M.; Dorjes  J.; de Wilde  P.; Duineveld  G.; Eleftheriou  A.; Herman  P. M. J.; Niermann  U.; Kingston  P.; Kunitzer  A.; Rachor  E.; Rumohr  H.; Soetaert  K.; Soltwedel  T. 《ICES Journal of Marine Science》1992,49(1):13-22

  相似文献   

花尾胡椒鲷养殖群体遗传多样性的等位酶研究   总被引：5，自引：1，他引：5  

丁少雄  王军  苏永全  全成干  张纹  郭丰  马梁 《台湾海峡》2001,20(2):224-227,T002

应用聚丙烯酰胺凝胶电泳（PAGE）技术对取自厦门火烧屿海水网箱的花尾胡椒鲷人工鱼养殖群体的遗传多样性进行等位酶分析。结果表明：所检测的16种同工酶的24个基因座位中，在XDH和GCDH基因座位上发现多态性，其平均多态位点比例为8．33％，基因座位有效等位基因的平均数Ne为1.083，平均杂合度的观测值Ho为0．00556，预期值He为0．00537,Hardy-Weinberg遗传偏离指数（D)为0．035。与其他鱼类相比较说明了其遗传多样性处于较低水平，文中对此进行了探讨并提出相应的种质资源管理保护措施。  相似文献   

多样性指数在海洋浮游植物研究中的应用   总被引：97，自引：12，他引：97  

孙军  刘东艳 《海洋学报》2004,26(1):62-75

对海洋浮游植物群落分析中常用的多样性指数进行了比较研究.对物种丰富度依赖型、丰度依赖型和实测浮游植物群落中物种丰富度、Shannon指数(以2或e为底)、Pielou均匀度指数、Simpson指数(1-D或1/D形式)、Margalef指数、Berger-Parker指数、McIntosh指数、McIntosh均匀度指数、Brillouin指数、Brillouin均匀度指数、Fisher α指数和Q统计指数等不同多样性指数计算结果进行了比较,发现不同多样性指数对浮游植物群落多样性的分析存在明显差异.对于一般情况下浮游植物群落多样性的研究,物种丰富度、Margalef指数、Fisher α指数、Shannon指数、Simpson相遇指数和Pielou指数的综合使用是较合适的,但对Margalef指数和Fisherα指数的结果要谨慎解释.并在综合应用各指数的基础上提出了浮游植物群落多样性分析的一般步骤.  相似文献   

岱海水体及沉积物细菌多样性及群落组成特征     

张雨晴  邵克强  胡洋  丁艳青  张恩楼  高光  汤祥明 《湖泊科学》2022,34(6):2070-2082

采用高通量测序技术,研究了内蒙古岱海流域入湖河流、湖水及沉积物细菌多样性及群落组成.结果显示,细菌多样性从高到低依次为：沉积物>河流>湖泊.聚类分析表明入湖河流、湖水和沉积物细菌群落可分为明显不同的3支,说明这3种生境中细菌群落结构有较大差异.物种注释结果表明,河流中优势细菌菌群为髌骨细菌（Patescibacteria）、变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）和放线菌门（Actinobacteria）;湖水中优势细菌菌群为放线菌门（Actinobacteria）;而沉积物中优势细菌菌群为变形菌门和绿弯菌门（Chloroflexi）.典范对应分析及Monte Carlo检验表明,电导率和悬浮物含量对水体中（河流与湖泊）细菌群落影响显著,二者共解释了细菌群落变化的86.5%;而冗余分析及Monte Carlo检验表明,泥深、磁化率和总有机碳对沉积物中细菌群落影响显著,三者共解释了细菌群落变化的47.9%.近30年来,岱海地区气候变化和人类活动导致湖水咸化,沉积物碳氮指标显著增长.岱海水体及沉积物细菌多样性及群落组成的差异及其主要驱动因子,反映了细菌对这种气候变化和人类活动共同作用的响应.  相似文献   

Characterization of a new lectin involved in the protoplast regeneration of <Emphasis Type="Italic">Bryopsis hypnoides</Emphasis>     

牛建峰  王广策  吕芳  周百成  彭光 《中国海洋湖沼学报》2009,27(3):502-512

A group of coenocytic marine algae differs from higher plants,whose totipotency depends on an intact cell(or protoplast).Instead,this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals.It is thought that lectins play a key role in the aggregation process.We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides.The lectin was ca.27 kDa with a pI between pH 5 and pH 6.The absence of carbohydrate suggested that the lectin was ...  相似文献   

Distribution patterns of interstitial polychaetes in sandy beaches of southern Brazil   总被引：1，自引：0，他引：1  

Maikon Di Domenico  Paulo da Cunha Lana  & André R. S. Garraffoni 《Marine Ecology》2009,30(1):47-62

This study describes the distribution patterns of interstitial polychaetes along morphodynamic gradients on six exposed sandy beaches in Santa Catarina and Paraná (South Brazil). Three random transects were sampled at two points on each beach, one at the swash and another at the surf zone, in winter and summer conditions. Six sediment replicates were collected at each sampling point using a corer of 4.6 cm internal diameter that removed 10 cm into the sediment. Abundance and composition of interstitial polychaete were correlated to wave height, slope, grain size, CaCO₃, chlorophyll a , omega indexes, temperature and relative tide range using a canonical correspondence analysis (CCA). A factorial ANOVA showed that taxa richness, mean density and Shannon's diversity were significantly higher at the reflective beaches, but average values differ significantly between transects and these differences change according to the beach zones on both sampling dates. PERMANOVA showed that polychaete associations differ among transects according to the beach zones. The composition of interstitial polychaete associations was significantly correlated to beach morphodynamics and features (P < 0.01). Polychaete associations of reflective beaches were more diverse than in other morphodynamic states. Intermediate beaches may also sustain diverse associations due to temporal variability of the morphodynamic patterns. Beaches presenting extreme dissipative morphodynamics and compacted sediments appear to be unfavourable for the occurrence of interstitial polychaetes.  相似文献   

Gradients in coral reef communities exposed to muddy river discharge in Pohnpei,Micronesia     

Yimnang Golbuu  Katharina Fabricius  Steven Victor  Robert H. Richmond 《Estuarine, Coastal and Shelf Science》2008

This study analyzed how coral communities change along a gradient of increasing exposure to a mud-discharging river in the Enipein Catchment, Pohnpei, Micronesia. Using video transects, we quantified benthic communities at five sites along a gradient moving away from the river mouth towards the barrier reef. The most river-impacted site was characterized by a high accumulation of mud, low coral cover and low coral diversity. Although coral cover leveled off at ∼400 m from the river mouth to values found at the outer-most sites, coral diversity continued to increase with increasing distance, suggesting that the most distant site was still impacted by the river discharges. Fungiidae, Pavona, Acropora, Pachyseris and Porites rus all significantly increased in cover with distance from the river, while Turbinaria decreased. The combined presence and abundance of these six species groups, together with coral species richness, may help to indicate the effects of terrestrial runoff in similar runoff-exposed settings around Micronesia, whereas coral cover is not a sensitive indicator for river impact. Coral reefs are important resources for the people of Pohnpei. To prevent further degradation of this important resource, an integrated watershed approach is needed to control terrestrial activities.  相似文献